Part:BBa_K3726044:Design

BOH_1_A_LV1

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 1230
  Illegal EcoRI site found at 1850
  Illegal PstI site found at 18
  Illegal PstI site found at 1755
  Illegal PstI site found at 2003
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 1230
  Illegal EcoRI site found at 1850
  Illegal PstI site found at 18
  Illegal PstI site found at 1755
  Illegal PstI site found at 2003
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 1230
  Illegal EcoRI site found at 1850
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 1230
  Illegal EcoRI site found at 1850
  Illegal PstI site found at 18
  Illegal PstI site found at 1755
  Illegal PstI site found at 2003
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 1230
  Illegal EcoRI site found at 1850
  Illegal PstI site found at 18
  Illegal PstI site found at 1755
  Illegal PstI site found at 2003
  Illegal NgoMIV site found at 1494
  Illegal NgoMIV site found at 1594
  Illegal NgoMIV site found at 1900
  Illegal AgeI site found at 594
  Illegal AgeI site found at 2272
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

The part is a MoClo Lv.1 part, assembled in in the acceptor Lv1 entry vector whose ori is “BBa_K2560036”, and it's resistance cassette is “BBa_K3726039” (MARK_Lv0_AmpR). This part has been assembled following the marburg collection standard, then the transcriptional unit is flanked by the part “BBa_K3726104” (5CON1(H)_NS1(mod)-up (PCC 11801)) that allows homologous recombination within the PCC 11801 Genome, and by the part 3'Con1 “BBa_K2560070” that will allow the assembly of a Lv2 construct.

Source

This construct has been made by golden gate reaction.

References

X. Liu, R. Miao, P. Lindberg and P. Lindblad, "Modular engineering for efficient photosynthetic biosynthesis of 1-butanol from CO2in cyanobacteria", 2021.